复方肝宁颗粒的制备及其对CCl诱导小鼠急性肝损伤的保护作用

刘钰华; 叶少武; 陈伟坚; 熊万娜; 罗善友; 黎柱德; 梁健钦; 陆声宇

doi:10.13422/j.cnki.syfjx.2016140018

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复方肝宁颗粒的制备及其对CCl诱导小鼠急性肝损伤的保护作用

药剂与炮制 | 更新时间：2024-01-04

- 复方肝宁颗粒的制备及其对CCl诱导小鼠急性肝损伤的保护作用
- Preparation of Compound Ganning Granules and Investigation of Its Protective Effect on Acute Hepatic Injury Induced by CCl in Mice
- 中国实验方剂学杂志 2016年22卷第14期页码：18-22
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西省自然科学基金项目（2014GXNSFAA118198）;广西中医药民族医药传承创新专项（GZZJ14-20）;广西高校科学技术研究项目（KY2015ZD060）
- DOI：10.13422/j.cnki.syfjx.2016140018
  中图分类号： R283.6;R285.5
- 纸质出版日期：2016
- 稿件说明：
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刘钰华, 叶少武, 陈伟坚, 等. 复方肝宁颗粒的制备及其对CCl诱导小鼠急性肝损伤的保护作用[J]. 中国实验方剂学杂志, 2016,22(14):18-22.

LIU Yu-hua, YE Shao-wu, CHEN Wei-jian, et al. Preparation of Compound Ganning Granules and Investigation of Its Protective Effect on Acute Hepatic Injury Induced by CCl in Mice[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(14): 18-22.
刘钰华, 叶少武, 陈伟坚, 等. 复方肝宁颗粒的制备及其对CCl诱导小鼠急性肝损伤的保护作用[J]. 中国实验方剂学杂志, 2016,22(14):18-22. DOI： 10.13422/j.cnki.syfjx.2016140018.

LIU Yu-hua, YE Shao-wu, CHEN Wei-jian, et al. Preparation of Compound Ganning Granules and Investigation of Its Protective Effect on Acute Hepatic Injury Induced by CCl in Mice[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(14): 18-22. DOI： 10.13422/j.cnki.syfjx.2016140018.

摘要

目的：将复方肝宁经验方改制成复方肝宁颗粒，优化其提取工艺并评价该制剂对四氯化碳（CCl4）诱导的小鼠急性肝损伤的保护作用。方法：通过Taguchi设计优化处方中五味子的乙醇提取工艺，正交试验优化其余药材的水提工艺，采用湿法制粒工艺得到复方肝宁颗粒；将60只昆明种小鼠，随机分成6组（正常组，CCl4模型组，联苯双酯阳性组和复方肝宁颗粒高、中、低剂量组），用0.1%CCl4复制小鼠急性肝损伤模型，测定小鼠血清中丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性，肝组织中超氧化物歧化酶（SOD）和丙二醛（MDA）的含量。结果：最佳醇提工艺为加12.5倍量75%乙醇回流提取2次，每次0.5h；最佳水提工艺为加12倍量水提取3次，每次1.5h；五味子醇甲及丹参素钠转移率均>95%。与正常组比较，模型组小鼠血清中AST和ALT的活性明显升高，肝组织中SOD活性降低，MDA含量升高；与模型组比较，复方肝宁颗粒高、中、低剂量组均能显著降低CCl4急性肝损伤小鼠血清中AST和ALT的活性，降低肝匀浆中MDA的含量，增强SOD的活性。结论：优选的提取工艺稳定可行。复方肝宁颗粒通过下调ALT，AST和MDA水平，上调SOD水平，对CCl4造成的小鼠急性肝损伤具有显著的保护作用。

Abstract

Objective: To prepare compound Ganning granules

optimize its extraction process and investigate its protective effect on acute hepatic injury induced by CCl4 in mice. Method: Ethanol extraction process of Schisandrae Chinensis Fructus was optimized by Taguchi design

water extraction process of the other herbs was optimized by orthogonal test.Compound Ganning granules was prepared through wet granulation process.Mice were randomly divided into six groups

included normal group

model group

bifendate group and high

middle

low dose group of compound Ganning granules.Acute hepatic injury mice model was induced by 0.1% CCl4.Levels of alanine aminotransferase (ALT)

aspartate aminotransferase (AST) in serum and malonialdehyde (MDA)

superoxide dismutase (SOD) in live tissue were detected. Result: After being optimized

transfer rates of schisandrin A and sodium danshensu were more than 90%.Compared with the normal group

activities of ALT

AST and level of MDA in model group were significantly increased and activity of SOD was inhibited.Compared with the model group

activities of ALT

AST and level of MDA in compound Ganning granules groups were significantly decreased and activity of SOD was increased. Conclusion: These optimized processes are stable and feasible.Compound Ganning granules has hepatoprotective effect on acute hepatic injury induced by CCl4 in mice by up-regulation of activity of SOD and down-regulation of activities of ALT

AST and MDA.

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