Objective: Combining an HPLC fingerprint with bioassay to make a quality evaluation of Tongtian oral liquid. Method: Fingerprint of Tongtian oral liquid was established by the HPLC method

and quantitative analysis of ten batches of Tongtian oral liquid was performed.Anti-platelet aggregation activity as biological activity indicator was used to determine the contribution of the active ingredients in Tongtian oral liquid. Result: Similarity among ten batches was more than 0.96

nineteen common peaks were obtained in the fingerprint of ten batches of Tongtian oral liquid.Compared with reference substance

No.6 peak was paeoniflorin

No.7 was prim-O-glucosylcimifugin

No.8 was liquiritin

No.10 was 4'-O-β-D-glucosyl-5-O-meth-ylvisammioside and No.11 was senkyunolide Ⅰ.Quantitative analysis showed that five ingredients had great differences among the ten batches of Tongtian oral liquid.Meanwhile

the anti-platelet aggregation activity showed that senkyunolide Ⅰ>4'-O-β-D-glucosyl-5-O-meth-ylvisammioside>prim-O-glucosylcimifugin>paeoniflorin

and senkyunolide Ⅰ was its main active ingredient(P<0.001). Conclusion: Fingerprint of Tongtian oral liquid associated with its efficacy has been established.Senkyunolide I

4'-O-β-D-glucosyl-5-O-meth-ylvisammioside and prim-O-glucosylcimifugin are the main chemical ingredients of Tongtian oral liquid related to the anti-platelet aggregation activity

which would provide a more reasonable means for the quality assessment of Tongtian oral liquid.