Objective: To establish the UPLC fingerprint for Bletilae Rhizoma from different origins

and provide experimental basis for effective overall quality control of Bletilae Rhizoma. Method: Fingerprint of Bletilae Rhizoma was developed by using UPLC-PDA

and the Acquity UPLC BEH C18 column (2.1 mm ×150 mm

1.7 μm) was used with acetonitrile-water solution as the mobile phase for gradient elution. The flow rate was 0.3 mL ·min-1; the column temperature was 45 ℃

and the detection wavelength was at 280 nm. Similarity evaluation

clustering analysis and principal components analysis (PCA) were used to evaluate the quality of Bletilae Rhizoma from different origins. Result: The 20 common peaks were marked in the fingerprint of Bletilae Rhizoma

and the chemical compounds of 9 common peaks were identified. The similarity of 43 batches of samples was 0.540-0.942

and they could be classified into two clusters by clustering analysis and four clusters by PCA. Six compounds in the fingerprint were found to cause differences. Conclusion: Establishment of UPLC fingerprint of Bletilae Rhizoma and the application of chemical pattern recognition can provide more comprehensive reference for the quality control of the herb.