Objective: To study the chemical constituents of dichloromethane extract from the leaf of Celastrus monospermus and to clarify the basis of its efficacy substance. Method: Methanol extracts from the leaf of C. monospermus were obtained by using dichloromethane

and purified by gradient elution on silica gel column chromatography with petroleum ether-ethyl acetate system. Their structures were identified by their physical and chemical properties

as well as the spectral data. Result: Eleven compounds were isolated and identified as monosperme A (1)

syringic acid (2)

syringaldazin (3)

vanillic acid (4)

β-sitosterol (5)

daucosterol(6)

usu-12-en-3-one-28-methyl (7)

α

β-amyrin (8)

3-oxo-olean-12-en-29-oic acid (9)

tripterfrielanon A (10)

and 3α

28-dihydroxy-olean-12-en-29-oic acid (11). Conclusion: The 11 compounds were obtained. Compound 1 was a new compound and other compounds were separated from the leaf of this plant for the first time.