Objective: To observe the effects of Fuzheng Kang'ai (FZKA) decoction on apoptosis of H1650 cells and discuss its molecular mechanism in apoptosis of H1650 cells. Method: Human lung adenocarcinoma cells (H1650 cells) were used as the research objects and treated for 24

48

72 h with 0.5

1.0

1.5

2.0

2.5

3.0 g · L-1 FZKA decoction

and a blank group was set up

then cell proliferation was detected by 3- (4

5-diethylthiazol-2-yl)-5- (3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-etrazolium

inner salt (MTS) assay. H1650 cells were treated for 24 h with 0.5

1.0

1.5 g · L-1 FZKA decoction and a blank group was set up

then cell apoptosis was detected by Annexin V-FITC/PI flow cytometry analysis. H1650 cells were treated for 24 h with 0.5

1.0

2.0 g · L-1 FZKA decoction and a blank group was set up

then vitality of Caspase-3/7 was detected by Caspase-3/7 vitality test kit. Simultaneously

effects of FZKA decoction on the expression levels of proCasapse-3

PARP and Bax were detected by Western blot assay. Result: As compared with the blank group

proliferation of H1650 cells was significantly inhibited by FZKA decoction in concentration-dependent and time-dependent manners (P<0.05). As compared with the blank group

early apoptosis of H1650 cells was significantly induced and vitality of Caspase-3/7 was increased by FZKA decoction in a concentration-dependent manner (P<0.05). As compared with the blank group

the expression levels of proCasapse-3 and PARP were significantly reduced by FZKA decoction in a concentration-dependent manner (P<0.05)

and the expression of Bax was increased by FZKA decoction in a time-dependent manner (P<0.05). Conclusion: Apoptosis is induced by FZKA decoction in way of activating Caspase-3 and Bax in H1650 cells.