大蒜素对胰腺癌细胞上皮间质化的影响

贺川; 马笛; 巩平; 黄伟

doi:10.13422/j.cnki.syfjx.2016140130

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大蒜素对胰腺癌细胞上皮间质化的影响

药理 | 更新时间：2024-01-04

- 大蒜素对胰腺癌细胞上皮间质化的影响
- Allicin Inhibits Epithelial-mesenchymal Transition of Human Pancreatic Cancer PANC-1 Cells
- 中国实验方剂学杂志 2016年22卷第14期页码：130-134
- 作者机构：
- 作者简介：
- 基金信息：
  
  上海市重点实验室开放课题项目（PC-2013-02）
- DOI：10.13422/j.cnki.syfjx.2016140130
  中图分类号： R285
- 纸质出版日期：2016
- 稿件说明：
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贺川, 马笛, 巩平, 等. 大蒜素对胰腺癌细胞上皮间质化的影响[J]. 中国实验方剂学杂志, 2016,22(14):130-134.

HE Chuan, MA Di, GONG Ping, et al. Allicin Inhibits Epithelial-mesenchymal Transition of Human Pancreatic Cancer PANC-1 Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(14): 130-134.
贺川, 马笛, 巩平, 等. 大蒜素对胰腺癌细胞上皮间质化的影响[J]. 中国实验方剂学杂志, 2016,22(14):130-134. DOI： 10.13422/j.cnki.syfjx.2016140130.

HE Chuan, MA Di, GONG Ping, et al. Allicin Inhibits Epithelial-mesenchymal Transition of Human Pancreatic Cancer PANC-1 Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(14): 130-134. DOI： 10.13422/j.cnki.syfjx.2016140130.

摘要

目的：探讨大蒜素对胰腺癌细胞上皮间质转化（EMT）的影响。方法：常规培养胰腺癌细胞株PANC-1细胞，分别以10μg·L-1转化生长因子-β1（TGF-β1），10μg·L-1TGF-β1+5mg·L-1大蒜素，5mg·L-1大蒜素处理PANC-1细胞，另设空白组，镜下观察细胞形态学改变。细胞划痕实验检测大蒜素是否能降低胰腺癌细胞的迁移能力；免疫荧光细胞化学法及免疫印迹法（Western blot）检测各组钙黏附蛋白-E（E-cadherin），波纹蛋白（Vimentin）的表达变化。结果：TGF-β1能够刺激PANC-1细胞发生典型的EMT形态变化，并伴有E-cadherin表达的上调及Vimentin的下调。划痕实验表明大蒜素能降低胰腺癌PANC-1细胞的迁移能力。免疫荧光细胞化学法及Western blot结果显示，与空白组及TGF-β1组比较，大蒜素组的E-cadherin表达明显上调（P<0.05），Vimentin明显下调（P<0.05），这表明大蒜素具有抑制PANC-1细胞产生EMT的效应。结论：大蒜素可抑制胰腺癌细胞发生EMT，进而可能在抑制肿瘤浸润与转移过程中发挥重要作用。

Abstract

Objective: To investigate the effect of allicin on epithelial-mesenchymal transition (EMT) of pancreatic cancer cells. Method: The PANC-1 cells were cultured and treated with 10 μg · L-1 transforming growth factor-β1 (TGF-β1)

10 μg · L-1 TGF-β1+5 μg · L-1 allicin

5 μg · L-1 allicin

respectively. Untreated cells were used for the normal control. Morphological changes were observed under inverted microscope. Wound scratch assay were performed to further identify if allicin could reduce the migration ability of PANC-1 cells. The expressions of E-cadherin and Vimentin in pancreatic cancer cells were detected by immunocytochemistry and Western blot. Result: The TGF-β1 can make PANC-1 cells show typical EMT morphological changes

which were accompanied by up-regulation of E-cadherin expression and down-regulation of Vimentin. Wound scratch assay indicated that allicin could reduce the migration ability of PANC-1 cells. According to the results of immunocytochemistry and Western blot

compared with the control group and the TGF-β1 group

the protein expression of E-cadherin was up-regulated in the allicin group (P<0.05)

and that of aimentin was down-regulated (P<0.05). Conclusion: Allicin can inhibit the EMT of human pancreatic cancer PANC-1 cells

and may play an important role in the inhibition of tumor invasion and metastasis.

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