Objective: To explore Arisaematis Rhizoma polysaccharide (ARPS) on the proliferation

apoptosis and Wnt/β-catenin signaling pathway of human renal cell carcinoma cell line GRC-1. Method: After entering into the logarithmic growth phase with RPMI-1640 culture fluid under routine condition

the GRC-1 cells were treated with different doses of ARPS (0

20

50

100

200 mg · L-1). The 0 mg · L-1 ARPS group was the blank group. The inhibition rates of proliferation at 24

48

72 and 96 h after the treatment were measured by viable cell counting kit assay (CCK-8). The apoptosis rate and cell cycle at 48 h after the treatment were detected by Annexin-FITC/PI double staining and PI staining methods via flow cytometry

respectively. Western blot was employed to detect the expression level of β-catenin in Wnt/β-catenin signaling pathway and its downstream target molecule C-myc and Cyclin D1 at 48 h after the treatment. Result: Compared with the blank group

ARPS within the range of 20-200 mg · L-1 presented an inhibitory effect on GRC-1 cell proliferation. The proliferation inhibition rate increased with the rise in time and concentration of ARPS in a dose-and time-dependent manner

and the above differences were statistically significant (P<0.05). Compared with the 0 μg · mL-1 ARPS

the early apoptosis rate of 50-200 mg · L-1 ARPS

the late apoptosis rate of 20-200 mg · L-1 and the total apoptosis rate were all elevated(P<0.05). There was a higher G0/G1 phase ratio than 20

50

100

200 mg · L-1 ARPS groups

and lower S and G2/M phase and β-catenin

C-myc and Cyclin D1 protein levels than 0 mg · L-1 (P<0.05). Indexes within the concentration range between 20-200 mg · L-1 were statistically significant(P<0.05). Conclusion: ARPS inhibited the proliferation of renal cell carcinoma cell line GRC-1

and induced cell apoptosis and G0/G1 arrest

with the effect of inhibiting the activation of Wnt/β-catenin signaling.