消癌平注射液HPLC指纹图谱及7种甾体有效成分的含量测定

龚晓斌; 曾庆花; 张凤; 高守红; 陆文铨; 陈万生

doi:10.13422/j.cnki.syfjx.2016150068

您当前的位置：

首页 >

文章列表页 >

消癌平注射液HPLC指纹图谱及7种甾体有效成分的含量测定

化学与分析 | 更新时间：2024-09-23

- 消癌平注射液HPLC指纹图谱及7种甾体有效成分的含量测定
- Fingerprint Analysis of XiaoaipingInjection and Simultaneous Determination of Its Seven Active Steroidal Components in XiaoaipingInjection by HPLC-ELSD
- 中国实验方剂学杂志 2016年22卷第15期页码：68-72
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81202866）
- DOI：10.13422/j.cnki.syfjx.2016150068
  中图分类号： O657.72;R286.0
- 纸质出版日期：2016
- 稿件说明：
移动端阅览
龚晓斌, 曾庆花, 张凤, 等. 消癌平注射液HPLC指纹图谱及7种甾体有效成分的含量测定[J]. 中国实验方剂学杂志, 2016,22(15):68-72.

GONG Xiao-bin, ZENG Qing-hua, ZHANG Feng, et al. Fingerprint Analysis of XiaoaipingInjection and Simultaneous Determination of Its Seven Active Steroidal Components in XiaoaipingInjection by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 68-72.
龚晓斌, 曾庆花, 张凤, 等. 消癌平注射液HPLC指纹图谱及7种甾体有效成分的含量测定[J]. 中国实验方剂学杂志, 2016,22(15):68-72. DOI： 10.13422/j.cnki.syfjx.2016150068.

GONG Xiao-bin, ZENG Qing-hua, ZHANG Feng, et al. Fingerprint Analysis of XiaoaipingInjection and Simultaneous Determination of Its Seven Active Steroidal Components in XiaoaipingInjection by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(15): 68-72. DOI： 10.13422/j.cnki.syfjx.2016150068.

摘要

目的：建立消癌平注射液HPLC指纹图谱，并准确测定消癌平注射液中tenacissoside H（TSH）等7种甾体有效成分的含量。方法：采用Agilent Eclipse XDB-C18色谱柱，流动相乙腈-水，梯度洗脱，柱温30℃，流速0.8 mL·min-1，平衡时间10 min，进样20 μL，蒸发光散射检测器漂移管温度60℃，载气压力0.4 MPa，检测17β-tenacigenin B（17β-TB），tenacigenin B（TB），tenacigenin A（TA），tenacigenoside A（TSA），marsdenoside I（MSI）和tenacissoside F（TSF）的含量；并运用《中药色谱指纹图谱相似度评价系统》软件建立消癌平注射液的对照指纹图谱，计算其相似度。结果： 11批消癌平注射液的HPLC指纹图谱有13个共有峰，相似度均在0.94以上。TSH等7种甾体在标准曲线范围内均呈现良好的线性（r>0.999），精密度、重复性和准确度均符合要求。结论：所建立的HPLC-ELSD方法操作简便、结果可靠，具有良好的精密度、稳定性和重复性，结合指纹图谱研究为消癌平注射液中多成分含量测定和质量控制提供了依据。

Abstract

Objective: To develop and validate a high performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD) method for the quantitative determination and fingerprint analysis of 7 active steroidal components in Xiaoaiping injection. Method: Chromatographic separation was performed on an Eclipse XDB-C18column (4.6 mm×250 mm

5 μm) by gradient elution using acetic acid in acetonitrile solution at a flow rate of 0.8 mL·min-1. The drift tube temperature of ELSD was set to 60℃ and nebulizer gas pressure was 0.4 MPa. Method validation was performed to assure its linearity

limits of detection and quantification

precision

repeatability

stability and accuracy. Result: Fingerprint analysis obtained 13 common peaks in Xiaoaiping injection

and the similarity was higher than 0.94. The HPLC-ELSD method allowed the quantification of 7 compounds (17β-tenacigenin B

tenacigenin B

tenacigenin A

tenacigenoside A

marsdenoside I and tenacissoside F)

and was successfully applied to fingerprint analysis for 11 batches of Xiaoaiping injection. Conclusion: It was the first time to apply HPLC-ELSD for simultaneous determination of 7 active ingredients and fingerprint analysis of Xiaoaiping injection. The results proved the reliability and comprehensiveness of the combination of quantitative analysis for marker ingredients and chemical fingerprint for TCM formula.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于多成分测定的贞芪扶正颗粒质量控制

糖脉康颗粒HPLC-ELSD指纹图谱

指纹图谱法及紫外光谱法比较鸦胆子油与多种食用油

基于HPLC指纹图谱的浙贝母质量评价

金边土鳖HPLC指纹图谱及氨基酸含量测定