Objective: To investigate effect of Scutellariae Radix on endogenous metabolites in the serum and liver of normal mice based on metabolomics. Method: 1H-NMR based on metabolomics

coupled with multivariate and univariate statistical analysis

was used to compare differential endogenous metabolites in the serum and liver between the normal mice and those treated by Scutellariae Radix. Result: Endogenous metabolism of mice treated by Scutellariae Radix had significant changes.Compared with the blank group

contents of 10 kinds of metabolites in serum had changed

contents of 3-hydroxybutyric acid

citric acid and choline increased

levels of α-glucose and β-glucose decreased in mice serum of Scutellariae Radix group;19 kinds of metabolites in the mice liver of Scutellariae Radix group also changed

levels of glutamic acid

isoleucine and leucine increased

levels of xanthine decreased.Changes of these metabolites in mice of Scutellariae Radix group involved in glycometabolism

lipid metabolism and amino acid metabolism. Conclusion: Impact of Scutellariae Radix on metabolism of mice is related with the cold property of Scutellariae Radix

its anti-oxidative

hepatoprotective and hypoglycemic effect and other pharmacological action.This study can provide a reference for the further research of property and pharmacological mechanism of Scutellariae Radix.