Objective: To explore the molecular mechanism of Shen's antihypertensive Siwu decoction treating hypertension. Method: By reference to Chinese Medicine Dictionary (second edition)

active ingredients of the four herbs (Uncariae Ramulus Cum Uncis

ALismatis Rhizoma

Chuanxiong Rhizoma

Raphani Semen) in Shen's antihypertensive Siwu decoction were obtained. The corresponding target proteins of the ingredients were retrieved in PubChem database. Meanwhile

hypertension genes were also retrieved in PubMed gene database. The protein and gene data were imported into the molecular network analysis platform to respectively build the corresponding molecular networks and biological pathways. Through online comparison

some targets and molecular network mechanisms for Shen's antihypertensive Siwu decoction treating hypertension can be found. Result: Shen's antihypertensive Siwu decoction showed a treatment effect on hypertension by regulating 3 molecules [nuclear factor-κB(NF-κB)

vascular endothelial growth factor(VEGF) and extracellular signal-regulated kinase 1/2(ERK1/2)] and 2 pathways (G-protein coupled receptor signaling and VEGF signaling). Conclusion: Shen's antihypertensive Siwu decoction is an effective prescription to treat hypertension. Molecular network analysis can predict the molecular mechanisms of traditional Chinese medicine compounds.