DNA gene identification is used as a more important molecular biological technology to identify traditional Chinese medicine in recent years. It can identify good herbs at the genetic level

with a high accuracy. DNA gene identification includes DNA molecule genetic markers

DNA hybridization and DNA barcoding molecular identification method

particularly the fastest growing one is DNA barcoding molecular identification method. DNA barcoding molecular identification method is a molecular biological technique to identify species using an admittedly short DNA sequence in genome

and an effective complement to traditional morphological identification methods. Because DNA sequences of different species were composed of four bases

namely adenine (A)

guanine (G)

cytosine (C) and thymine (T)

analysis on a specific sequence of DNA fragments can distinguish different species. Hebert

proposed the animal species identification method by measuring mitochondrial cytochrome C oxidase subunit I (COI) gene sequences in 2003.CHEN Shi-lin proposed to identify genes with COI gene-dominated and ITS2 gene-supplemented animal medicine DNA barcoding system on the basis of a large number of experiments on animals. A large number of experiments have showed that

DNA barcoding is accurate

feasible

simple and general to identify animals

and provides a new method for identifying animal species

finding new species and authenticating animal medicines. However

because the technology is to identify by gene

it has higher requirements for experimental materials and extraction methods. Particularly

animal medicines prepared many years ago have a severe DNA degradation

which sets higher requirements for the DNA extraction technology. In this case

the technology can only identify the exact source

but not its medicinal parts

which has to be identified in the combination with other identification methods. In this article

studies on DNA barcodes at home and abroad are summarized to provide a theoretical basis for future studies and applications.