Objective: To explore and confirm the bioequiavailability between Lonicerae Japonicae Flos and Lonicerae Flos

and provide a new solution for the dispute between them. Method: Three groups of rats were treated with 2 mL of Lonicerae Japonicae Flos extract

Lonicerae Flos extract and ultrapure water by oral administration

respectively.Blood taking time points were 0.25

0.5

1

1.5

2

3

4

6

8

12

24 h

then determined by HPLC and calculated pharmacokinetic parameters and spectral parameters by chromatokinetics formula. Result: Total amount of zero-order moment

the first and second-order moment of overall drug metabolism

the first and second-order moment of total amount of fingerprint characteristic peaks of Lonicerae Japonicae Flos were 1 621.014 μg·min·L-1

12.632 min

34.717 min2

16.088 min and 16.626 min2;these parameters of Lonicerae Flos were 1 769.339 μg·min·L-1

13.308 min

38.929 min2

16.569 min and 17.391 min2

respectively.These parameters between Lonicerae Japonicae Flos and Lonicerae Flos had no significant difference. Conclusion: Plasma concentration of Lonicerae Japonicae Flos and Lonicerae Flos can keep pace with the time

they have the same in vivo metabolic effects.