多指标加权评分法优选肛洗二号凝胶剂的提取工艺

庞晓晨; 刘志东; 黄瑞; 成睿珍; 赵静; 潘卫三

doi:10.13422/j.cnki.syfjx.2016160022

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多指标加权评分法优选肛洗二号凝胶剂的提取工艺

药剂与炮制 | 更新时间：2024-01-04

- 多指标加权评分法优选肛洗二号凝胶剂的提取工艺
- Optimization of Extraction Technology of Gangxi Ⅱ Gels by Multi-index Weighted Scoring Method
- 中国实验方剂学杂志 2016年22卷第16期页码：22-24
- 作者机构：
- 作者简介：
- 基金信息：
  
  天津市卫生和计划生育委员会中医中西医结合科研课题（2015144）
- DOI：10.13422/j.cnki.syfjx.2016160022
  中图分类号： R286.0
- 纸质出版日期：2016
- 稿件说明：
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庞晓晨, 刘志东, 黄瑞, 等. 多指标加权评分法优选肛洗二号凝胶剂的提取工艺[J]. 中国实验方剂学杂志, 2016,22(16):22-24.

PANG Xiao-chen, LIU Zhi-dong, HUANG Rui, et al. Optimization of Extraction Technology of Gangxi Ⅱ Gels by Multi-index Weighted Scoring Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 22-24.
庞晓晨, 刘志东, 黄瑞, 等. 多指标加权评分法优选肛洗二号凝胶剂的提取工艺[J]. 中国实验方剂学杂志, 2016,22(16):22-24. DOI： 10.13422/j.cnki.syfjx.2016160022.

PANG Xiao-chen, LIU Zhi-dong, HUANG Rui, et al. Optimization of Extraction Technology of Gangxi Ⅱ Gels by Multi-index Weighted Scoring Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 22-24. DOI： 10.13422/j.cnki.syfjx.2016160022.

摘要

目的：为了更好地保证肛洗二号凝胶剂的临床疗效和质量控制，优选其提取工艺。方法：采用UPLC测定芦丁、盐酸小檗碱和槲皮素的含量，流动相乙腈-0.1%甲酸梯度洗脱，流速0.3 mL·min-1，检测波长260 nm。以盐酸小檗碱、芦丁和槲皮素提取量的综合评分为评价指标，通过单因素试验和正交试验考察提取次数、提取时间和溶剂用量对提取工艺的影响。结果：最佳提取工艺为加10倍量水提取3次，每次1 h。在该工艺条件下，芦丁、盐酸小檗碱、槲皮素平均提取量分别为9.154，1.264，0.734 mg·g-1。结论：优选的提取工艺稳定、可靠，为肛洗二号凝胶剂的进一步开发利用提供实验依据。

Abstract

Objective: To optimize extraction conditions of Gangxi Ⅱ gels. Method: UPLC was employed to determine contents of berberine hydrochloride

rutin and quercetin in gels with mobile phase of acetonitrile-0.1%formic acid solution for gradient elution

flow rate of 0.3 mL·min-1 and detection wavelength at 260 nm.Taking composite score of extracting amounts of berberine hydrochloride

rutin and quercetin as index

orthogonal test and single factor tests were adopted to optimize extraction process. Result: Optimum extraction technology was:extracted thrice with 10 times the amount of water for 1 hour of per time;under these conditions

extracting amounts of rutin

berberine hydrochloride and quercetin were 9.154

1.264

0.734 mg·g-1

respectively. Conclusion: This optimized technology is stable and feasible

which offers expermental basis for further development of Gangxi Ⅱ gels.

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