Objective: To establish the content determination method for flavonoids ingredients (quercetin-3-rutinoside)in Guizhou Miao medicine Laportea bulbifera

find out the dominant environmental factors which impact the original differences of quality in Guizhou Miao medicine L. bifera by gray correlation analysis

and discuss the influence of environmental factors on formation mechanism of quality in medicinal materials L. bifera. Method: HPLC method was employed to measure the difference of quercetin-3-rutinoside content of Guizhou Miao medicine L. bifera from different producing areas

with acetonitrile-methyl alcohol-water (15:15:70) as the mobile phase. The wavelength was set at 360 nm. The ammonium nitrogen

available phosphorus

available potassium

pH and water in the soil of different producing areas were determined by soil nutrients tester; geographical climatic factors were obtained by consulting the meteorological department near the place of origin and by GPS and altitude meter. The correlation between flavonoids ingredient (quercetin-3-rutinoside) content and climate and soil factors was analyzed by grey correlation analysis. Result: The content of quercetin-3-rutinoside of L. bifera from different producing areaswas 0.094 6-4.969 mg·g-1 due to the great influence of geographical climate factors. Conclusion: The established determination method for flavonoids contents of medicinal material of L. bifera is easy

fast

accurate and feasible. It can be used as the quality control method for medicinal materials L. bifera. The dominant environmental factors were ensured by gray correlation analysis. It provides preliminary experimental basis for selection of excellent germplasm resources and GAP standardized planting. It also provides experimental basis to improve the quality standard of the medicinal materials of Guizhou Miao medicine L. bifera.