Objective: To study the isolation

puriffication and structural analysis of the polysaccharides from the Curculiginis Rhizoma. The work will provide the theoretical foundation for the further application of Curculiginis Rhizoma polysaccharides. Method: The crude polysaccharide was obtained by hot water extraction

ethanol precipitation and freezedrying. After further purification by DEAE-cellulose and Sepharose CL-6B column chromatography

pure polysaccharide (XMP-1) was achieved. The structural of XMP-1 was elucidated by GPC

UV

IR and GC-MS. Result: XMP-1 was composed of mannose

glucose

galactose with a molar ratio of 81.2:5.7:1. Total carbohydrate content of XMP-1 was 91.8% and the molecular weight was 3 031 Da. XMP-1 have no nucleic aicd

protein and pigment. IR chromatography showed that XMP-1 has pyrane characteristic absorption peak. XMP-1 in molecular conformation in aqueous solution can be naturally curly structure. Conclusion: XMP-1 is a kind of small molecular weight neutral pure heterosaccharides.