Objective: To establish a HPLC method for determination of the contents of vanillic acid

syringic acid

and ferulic acid in the Corallodiscus flabeilatus. Method: The contents of vanillic acid

syringic acid

and ferulic acid were detected under the following conditions:Agilent TC18 (4.6 mm×250 mm

5 μm) column

withacetonitrile (A)-0.2%phosphoric acid solution(B)as the mobile phase for gradient elution (0-22 min

10%-13%A; 22-30 min

13%A). The detection wavelength was 290 nm. The column temperature was 40℃

and flow rate was 1 mL·min-1. Result: Vanillic acid showed a good linear relationship within the range of 0.092-0.46 μg (r=0.999 7)

with an average recovery of 98.47%and RSD of 1.7%(n=6). Syringic acid showed a good linear relationship within the range of 0.092-0.46 μg (r=0.999 6)

with an average recovery of 98.07%and RSD of 1.9%(n=6). Ferulic acid showed a good linear relationship within the range of 0.073 6-0.368 μg (r=0.999 5)

with an average recovery of 98.58%and RSD of 1.4%(n=6). The results showed the content was 0.265 mg·g-1for vanillic acid

0.159 mg·g-1for syringic acid and 0.085 mg·g-1for ferulic acidin tested 6 batches of C. flabeilatus. Conclusion: The study showed that the HPLC method could detect the contents of vanillic acid

syringic acid

and ferulic acid in C. flabeilatus. This method is simple

highly specific

and reliable

which can be used for the quantitative control of C. flabeilatus.