萆薢除痹汤对大鼠尿酸性肾病防治作用的影响

江雪纯; 平凡; 张谨枫; 汪悦

doi:10.13422/j.cnki.syfjx.2016160103

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萆薢除痹汤对大鼠尿酸性肾病防治作用的影响

药理 | 更新时间：2024-01-04

- 萆薢除痹汤对大鼠尿酸性肾病防治作用的影响
- Effect of Bixie Chubi Decoction in Preventing and Treating Uric Acid Nephropathy
- 中国实验方剂学杂志 2016年22卷第16期页码：103-108
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家中医药管理局重点学科（中医痹症学）开放基金项目（BBXK2013101）;江苏省研究生创新工程项目（KYLX150989）
- DOI：10.13422/j.cnki.syfjx.2016160103
  中图分类号： R285.5
- 纸质出版日期：2016
- 稿件说明：
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江雪纯, 平凡, 张谨枫, 等. 萆薢除痹汤对大鼠尿酸性肾病防治作用的影响[J]. 中国实验方剂学杂志, 2016,22(16):103-108.

JIANG Xue-chun, PING Fan, ZHANG Jin-feng, et al. Effect of Bixie Chubi Decoction in Preventing and Treating Uric Acid Nephropathy[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 103-108.
江雪纯, 平凡, 张谨枫, 等. 萆薢除痹汤对大鼠尿酸性肾病防治作用的影响[J]. 中国实验方剂学杂志, 2016,22(16):103-108. DOI： 10.13422/j.cnki.syfjx.2016160103.

JIANG Xue-chun, PING Fan, ZHANG Jin-feng, et al. Effect of Bixie Chubi Decoction in Preventing and Treating Uric Acid Nephropathy[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(16): 103-108. DOI： 10.13422/j.cnki.syfjx.2016160103.

摘要

目的：研究萆薢除痹汤对尿酸性肾病大鼠肾组织核转录因子-κB（NF-κB），单核细胞趋化因子（MCP-1），细胞间黏附分子-1（ICAM-1），血管细胞间黏附分子-1（VCAM-1）的影响；研究萆薢除痹汤对细胞中以上炎症指标表达的调控作用；探讨萆薢除痹汤对尿酸性肾病防治的影响。方法：采用腺嘌呤（100 mg·kg-1）和盐酸乙胺丁醇（250 mg·kg-1）制作尿酸性肾病大鼠模型，设立空白组，模型组，西药别嘌醇组（5 mg·kg-1），萆薢除痹汤低、中、高剂量组（7.25，14.49，29.98 g·kg-1）6组，实验第21天处死，采用实时荧光定量PCR（Real-time PCR）法及免疫印迹法（Western bolt）检测各组大鼠肾脏组织中NF-κB，MCP-1，ICAM-1，VCAM-1的mRNA含量和蛋白表达水平。同时体外培养NR 8383巨噬细胞，各组加入不同浓度含药血清，将其分为空白组、模型组、中药低（2.5%），中（5%），高（10%）剂量组5组，培养24 h后提取NR8383细胞总蛋白，用Western bolt法检测以上各指标的表达。结果：体内实验发现，与正常组比较，萆薢除痹汤低、中、高剂量组NF-κB，MCP-1，ICAM-1，VCAM-1的蛋白及mRNA表达与模型组相比明显降低，萆薢除痹汤低、中、高剂量组与别嘌醇比较，组间比较无统计学差异。体外实验发现萆薢除痹汤低、中、高浓度组各指标蛋白的表达与模型组相比降低（P<0.05），其中、高浓度组下调程度更明显（P<0.01）。结论：体内及体外实验均证实萆薢除痹汤可通过抑制NF-κB，MCP-1，ICAM-1，VCAM-1所导致的炎症反应而保护肾功能，起到防治尿酸性肾病的作用，其机制可能与其能减轻尿酸盐的沉积的有关。

Abstract

Objective: To observe the effect of Bixie Chubi decoction (BXCBD) on nuclear transcription factor-κB (NF-κB)

monocyte chemotactic factor-1 (MCP-1)

intercellular adhesion molecule-1 (ICAM-1)

vascular intercellular adhesion molecule-1 (VCAM-1)in renal tissues of rats with uric acid nephropathy

study on regulation of BXCBD on the expression of the above cell inflammatory index

and discuss about the mechanism of BXCBD in preventing and treating uric acid nephropathy renal protection. Method: Adenine (100 mg·kg-1) and ethambutol hydrochloride (250 mg·kg-1) were used to prepare the model of uric acid nephropathy rats. The rats were divided into six groups:normal group

model group

western medicine allopurinol group (5 mg·kg-1)

BXCBD low

medium and high-dose groups (7.25

14.49

29.98 g·kg-1). The rats was sacrificedatthe 21st day.The contents of NF-κB

MCP-1

VCAM-1

ICAM-1 and mRNA in kidney tissues of each group were detected by real-time PCR and Western bolt methods. Meanwhile

NR 8383 macrophages were cultured in vitro

each group wasgiven different concentrations of drug-containing serums

and divided into blank group

model group

traditional Chinese medicine (TCM) low-concentration (2.5%)

middle-concentration (5%) and high-concentration (10%)groups (n=5).NR8383 total cellular proteins were extracted after being cultured for 24 h. Western bolt method was used to detect the expression of the above indicators. Result: According to the in vivo experiment

compared with the normal group

the BXCBD low

medium and high-dose groups showed signficiantly lower NF-κB

MCP-1

ICAM-1 and VCAM-1 protein and mRNA expressions. Compared with allopurinol group

BXCBD low

medium and high-dose groups showed no inter-group statistical difference. On the basis of the in vitro experiment

compared with the model group

BXCBD low

medium and high-dose groups showed lower protein expressions (P<0.05)

particularly middle and high-concentration groups (P<0.01). Conclusion: In vivo and in vitro experiments have confirmed that Bixie Chubi decoction has the effect in preventing and treating uric acid nephropathy.By inhibiting NF-κB

MCP-1

ICAM-1

VCAM-1 in the inflammatory reaction. The mechanism may be related to the reduction of the deposition of uric acid salts.

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