Objective: To observe the protective effects of total flavonoids from Campsis Flos (CFTF) on cerebral ischemia reperfusion injury in rats. Method: Sixty SPF grade Wistar rats were randomly divided into sham operation group

model group

positive drug group (aspirin

50 mg·kg-1)

CFTF high dose and low dose groups (200

100 mg·kg-1). Models of transient middle cerebral artery occlusion (tMCAO) were established in all the rats except those in sham operation group. The behavioral injury was detected by neurologic deficit scores (NDS); the cerebral histopathology was detected by HE staining; and the levels of neuron specific enolase (NSE) in serum

interleukin(IL)-10

and TGF-β1 in brain homogenate were detected by ELISA

to discuss the protective effect of CFTF on cerebral ischemia reperfusion injury. Result: As compared with sham operation group

the neurologic deficit scores were significantly higher in rats of model group (P<0.01)

with serious cerebral histopathology injuries; and the level of NSE in serum was significantly increased (P<0.01); the levels of IL-10

and TGF-β1 in brain homogenate were decreased dramatically (P<0.01). As compared with the model group

the neurologic deficit symptoms were significantly improved in CFTF high dose group (200 mg·kg-1)

with decreased neurologic deficit scores (P<0.05); cerebral histopathology injury was significantly decreased(P<0.01

P<0.05)

NSE level in serum was decreased (P<0.05)

and levels of IL-10

TGF-β1 in brain tissues were increased (P<0.01

P<0.05) in CFTF high dose and low dose groups (200

100 mg·kg-1) and aspirin group. Conclusion: CFTF had the protective effects on tMCAO rats by decreasing the level of NSE

increasing the levels of anti-inflammation cytokines (IL-10 and TGF-β1)

and attenuating the inflammation injury.