Objective: To evaluate the effects of alternol on the level of glycolysis in melanoma B16F0 cells of mice

and to explore its mechanism. Method: Mice melanoma B16F0 cells were cultured and treated with different concentrations of alternol in vitro. The proliferation of melanoma B16F0 cells was detected by a sulforhodamine B assay. The content of adenosine triphosphate (ATP) was detected by a reverse high performance liquid chromatography method

and the content of lactic acid in the medium was detected by a lactic acid assay kit. Glucose intake of B16F0 cell was determined by radioactive isotopes. The activities of hexokinase (HK)

pyruvate kinase (PK) and lactic dehydrogenase (LDH) were detected by the related assay kits. Result: Compared with the control group

alternol could inhibit the release of lactic acid and the production of ATP

reduce glucose uptake and the activities of PK and LDH in B16F0 cells(P<0.05

P<0.01). Conclusion: alternol inhibits glycolysis in B16F0 cells

which may be related to reducing the glucose uptake and the activities of PK and LDH.