Objective: To investigate the protective effects of resveratrol(Res) on pancreatic cell oxidative injury in gestational diabetic rats. Method: The 100 rats with gestation for five days were injected STZ (35 mg·kg-1) by intraperitonal to make gestational diabetic model

which were randomly devided into five groups:normal gestation group

gestational diabetic model group

Res(60

120

240 mg·kg-1) treated groups and metformin hydrochloride 200 mg·kg-1 treated group

which was 20 expremental animals every group. And selected 20 rats with gestation for five days as normal gestation control group and another 20 rats with no gestation as normal control group. Before the drugs were given and 7

14 days after treated

the level of plasma glucose and insulin were determined. Two weeks later

the content of malondialdehyde(MDA) and reactive oxygen species(ROS) were detected; the activity of superoxide dismutase(SOD)

glutathion peroxidase(GSH-Px)

catalase(CAT) in pancreatic tissue were detected

and the content of MDA was also detected; the histopathological changes of pancreatic tissue and pancreatic cells apoptosis were observed

and the apoptosis index were analysised. Result: Compared with normal control group

the level of plasma glucose and insulin of the normal gestation control group was not significantly different

the content of MDA

ROS in serum was not significantly different

the activity of SOD

GSH-Px

CAT and the content of MDA in pancreatic tissue were not significantly different

the level of pancreatic tissue histopathological changes and hepatic cells apoptosis were not significantly different. Compared with the normal gestation control group

the level of plasma glucose in gestational diabetic model group was significantly increased and the level of insulin was significantly decreased(P<0.01); the content of MDA

ROS in serum were significantly increased(P<0.01); the activity of SOD

GSH-Px

CAT in pancreatic tissue were significantly decreased and the content of MDA were significantly increased(P<0.05

P<0.01)

the pancreatic tissue histopathological changes and hepatic cells apoptosis were obviously improved

the AI were significantly increased(P<0.01). Compared with the estational diabetic model group

the content of MDA

ROS in serum in Res(60

120

240 mg·kg-1) treated groups were significantly decreased(P<0.05

P<0.01)

and the content of MDA in pancreatic tissue were significantly decreased(P<0.05

P<0.01); the level of plasma glucose in Res(120

240 mg·kg-1) treated groups were significantly decreased and the level of insulin was significantly increased(P<0.05

P<0.01)

the activity of SOD

CAT in pancreatic tissue were significantly increased(P<0.05

P<0.01)

the AI of pancreatic cell was significantly decreased (P<0.05

P<0.01); the activity of GSH-Px in pancreatic tissue of Res 240 mg·kg-1 treated group was significantly increased(P<0.05); and pancreatic tissue histopathological changes and hepatic cells apoptosis were significantly improved

especially the level of Res 240 mg·kg-1 treated group. Compared with the Metformin hydrochloride treated group

the level of insulin in Res 240 mg·kg-1 treated group was significantly increased(P<0.05)

the content of MDA

ROS in serum were significantly decreased(P<0.05)

the activity of SOD

GSH-Px

CAT in pancreatic tissue were significantly increased(P<0.05

P<0.01)

the AI was significantly decreased(P<0.01). Conclusion: Res could effectively improve insulin secretion

lowering blood sugar

improve antioxidant ability

reducing the damage of free radical

depress the pancreatic tissue histopathological changes and hepatic cells apoptosis

suggesting that Res had dose-dependent protective effects on pancreatic cell oxidative injury in gestational diabetic rats

and these effects of Res was better than Metformin hydrochloride except lowering blood sugar.