Objective: To provide the fundamental basis for determining suitable planting areas of Lonicerae Japonicae Flos by analyzing and comparing the effect of ecological factors on active ingredients of Lonicerae Japonicae Flos. Method: Totally 11 samples of Lonicerae Japonicae Flos were collected from different areas with altitudes ranging from 4-2100 m

10 bioactive contents of Lonicerae Japonicae Flos were obtained by HPLC. Meanwhile

climatic factors of different areas were collected

and the resulting data were statistically analyzed using SPSS 17.0 statistical software for the correlation analysis and the stepwise linear regression analysis. Result: There were significant differences among active ingredients of Lonicerae Japonicae Flos from different areas. Altitude is the dominant ecological factor influencing on the contents of chlorogenic acid and luteoloside. With the increase of altitude

the contents of chlorogenic acid and luteoloside showed a trend of increase. The content of caffeic acid was significantly positively related with the highest temperature in July

and negative related with the sunshine time. The content of isochlorogenic acid A and rainfall were significantly negative related

the content of quercetin and the minimum temperature in January showed a significant positive correlation. Conclusion: According to the results of this experimental study

we can speculate that with the increase of altitude in the planting areas of Lonicerae Japonicae Flos

the flowering temperature reduces

which is more conducive to the synthesis of chlorogenic acid and luteoloside.