CAO Yun-jiao, WANG Xu, RONG Rong. Ligustri LucidiFructus and Fruit of by Ultraviolet Spectrum Quad-Chemical Pattern Recognition[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(17): 32-36.
CAO Yun-jiao, WANG Xu, RONG Rong. Ligustri LucidiFructus and Fruit of by Ultraviolet Spectrum Quad-Chemical Pattern Recognition[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(17): 32-36. DOI: 10.13422/j.cnki.syfjx.2016170032.
Objective: To develop the method of Ultraviolet spectrum quad (UV)-chemical pattern recognition for the identification of Ligustri Lucidi Fructus and the fruit of Ligustrum japonicum. Method: Ligustri Lucidi Fructus and the fruit of L. japonicum were exact parallelly by four solvents including petroleum ether
methylene chloride
ethanol and water. Ultraviolet spectra of each fraction was scanned and the absorbance at different wavelengths was extracted at regular intervals for principle component analysis
Q-type clustering analysis and Fisher discrimination analysis. Result: Based on the results of principal component analysis and the Q-type clustering analysis
the discriminant function was established as follows:Y=-0.810+5.035Z1
p+3.645Z2
p-5.992Z3
p-5.609Z1
m+3.569Z2
m+6.519Z3
m-54.267Z1
e+6.901Z2
e+35.281Z3
e+13.334Z4
e+12.349 Z1
w-35.806.Z2
w. The accuracy of retrospective assessment was 100% in our experiment
and the UV-chemical pattern recognition method was established to identify Ligustri Lucidi Fructus and the fruit of L. japonicum. Conclusion: This method is scientific
accurate and simple
which can be used for the quick identification of Ligustri Lucidi Fructus and the fruit of L. japonicum.