Objective: To study the difference of chemical components in Ixeris sonchifolia (ISH) from different origins by UPLC-Q-TOF-MS. Method: The separation was performed on a Waters Acquity BEH C18 column (2.1 mm×100 mm

1.7μm) with acetonitrile-0.1%formic solution as the mobile phase for gradient elution. Mass spectrometric data were obtained in electrospray ionization (ESI) positive and negative modes. Meanwhile

the chemical components were identified with quasi-molecular ions and secondary fragment ions. Principal component analysis (PCA) and partial least squares discriminate analysis (PLS-DA) were used for multivariate statistical analysis. Result: Eighteen chemical components were identified from ISH. Among them

11

13α-dihydroixerin Z

luteolin

3

4-dihydroxybenzoic acid

apigenin-7-O-glucuronide

rutin

chicory acid

luteolin-7-O-glucuronide and guanosine were the main differential compositions of ISH from different origins. Conclusion: The difference chemical constituents in ISH from different origins were identified by the established UPLC-Q-TOF-MS-MS method

which could provide the basis for the quality control of ISH.