Objective: To study the HPLC fingerprint of the total alkaloids from different parts of the Sophora alopecuroides

and provide the basis for scientific evaluation and effective control of the quality of total alkaloids in S. alopecuroides. Method: Totally 16 batches of S. alopecuroides from different producing areas and the total alkaloids were used to gain fingerprints by HPLC-DAD. The obtained data was analyzed by similarity evaluation

and the pattern recognition was carried out by cluster analysis and principal component analysis. Result: Fingerprints of S. alopecuroides fructus and herba S. alopecuroides extracts were established; common peaks were determined

and the chromatographic peaks were identified. Through cluster analysis and principal component analysis

the total alkaloids extract of the 16 batches Chinese medicinal materials were divided into 3 groups according to different producing areas. Conclusion: S. alopecuroides fructus and herba S. alopecuroides extracts had good fingerprint features and specificity

which could be used for the overall quality control of the total alkaloids from S. alopecuroides fructus and herba S. alopecuroides.