Objective: To study the chemical constituents of Tibet medicine Corydalis hendersonii. Method: The chemical constituents were isolated through combined techniques

silica gel

Sephadex LH-20

preparative TLC and semi-preparative HPLC

and their structures were determined on the basis of physicochemical properties

MS and NMR spectroscopic analysis and literature data. Result: Eleven alkaloids were isolated from the total alkaloids part of ethanol extract from C. hendersonii. Their structures were identified as 9-methyldecumbenine C(1)

protopine(2)

allocrytopine(3)

stylopine(4)

tetrahydroberberine(5)

tetrhydropalmatine(6)

tetrahydrocolumbamine(7)

ochotensimine(8)

fumariline(9)

dihydrosanguinarine(10)

and berberine(11). Conclusion: Compounds 3 and 5-11 were obtained from this species for the first time.