Objective: To detect the relative content changes of endogenous metabolites in plasma of BALB/c mice by GC-MS technology

and to explore the mechanism of Jinxin oral liquid against respiratory syncytial virus (RSV) pneumonia in vivo. Method: BALB/c mice were challenged intranasally with RSV to establish the RSV pneumonia model

then intragastric administration of Jinxin oral liquid with dose of 27.6 g · kg-1 · d-1.After 3 days of inoculation

five mice in each group were hanged and inferior lobe of right lung were collected to take histopathological examination.After continuously given Jinxin oral liquid for 7 days

plasma were collected to detect the endogenous metabolites by GC-MS (m/z 50-500) and possible metabolic pathways concerned were analyzed. Result: Thirty-two endogenous metabolites in plasma were detected

arachidonic acid

glutamine

lactic acid

D-glucose

urea and 1

5-anhydro-D-sorbitol were differential metabolites among them.Metabolic pathways of archidonic acid

glutamine and pyruvate were the most concerned. Conclusion: The antiviral effect of Jinxin oral liquid against RSV pneumonia may be related to regulation of metabolic disorder caused by RSV.