Objective: To investigate the protection effect of Angelicae Sinensis Radix and Hedysari Radix polysaccharide (AHP) in acute myocardial ischemic rats. Method: Eighty male Wistar rats were randomly divided into sham operation group

myocardial ischemia group

3

6

12 g · kg-1 AHP groups

n=16 in each group. The acute myocardial ischemia models were established by ligating the left anterior descending coronary artery of the rats.3

6

12 g · kg-1 AHP was used by intragastric administration in the rats with 24 h of myocardial ischemia. Then the samples were collected from the ras at 36 hours

and 14 days after treatment respectively. Serum creatine kinase (CK)

creatine kinase MB (CK-MB)

lactate dehydrogenase (LDH)

and LDHI levels were detected by biochemical analyzer; the expressions of heat shock protein70(Hsp70)

Hsp32 and vascular endothelial growth factors (VEGF) mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). Result: As compared with the sham operation group

the electrocard iogram ST segment dynamics were increased in myocardial ischemia group(P<0.05); the contents of CK

CK-MB

LDH and LDHI were significantly increased in myocardial ischemia group at 36 h

and the expression levels of Hsp70

Hsp32 and VEGF mRNA were also incresaed either (P<0.05

P<0.01). As compared with the myocardial ischemia group

the contents of CK

CK-MB

LDH and LDH1 were significantly increased in various dose treatment groups at 36 h; and the expression levels of Hsp70

Hsp32 and VEGF mRNA were incresaed (P<0.05

P<0.01). Conclusion: The Angelicae Sinensis Radix and Hedysari Radix ultrafiltration substabce can protect myocardium on acute myocardial ischemia.