芍药汤高效液相色谱指纹图谱的建立

马大龙; 张晓平; 魏宝红; 张鹏; 马晓青; 孟凡刚; 刘红兵; 代龙

doi:10.13422/j.cnki.syfjx.2016180078

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芍药汤高效液相色谱指纹图谱的建立

化学与分析 | 更新时间：2024-01-04

- 芍药汤高效液相色谱指纹图谱的建立
- Establishment of HPLC Fingerprint of Shaoyao Tang
- 中国实验方剂学杂志 2016年22卷第18期页码：78-81
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2016180078
  中图分类号： O657.72;R284.1
- 纸质出版日期：2016
- 稿件说明：
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马大龙, 张晓平, 魏宝红, 等. 芍药汤高效液相色谱指纹图谱的建立[J]. 中国实验方剂学杂志, 2016,22(18):78-81.

MA Da-long, ZHANG Xiao-ping, WEI Bao-hong, et al. Establishment of HPLC Fingerprint of Shaoyao Tang[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 78-81.
马大龙, 张晓平, 魏宝红, 等. 芍药汤高效液相色谱指纹图谱的建立[J]. 中国实验方剂学杂志, 2016,22(18):78-81. DOI： 10.13422/j.cnki.syfjx.2016180078.

MA Da-long, ZHANG Xiao-ping, WEI Bao-hong, et al. Establishment of HPLC Fingerprint of Shaoyao Tang[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 78-81. DOI： 10.13422/j.cnki.syfjx.2016180078.

摘要

目的：建立芍药汤水煎液的RP-HPLC指纹图谱检测方法。方法：使用Agilent ZORBAX SB-C18色谱柱（4.6 mm×250 mm，5 μm），柱温25℃，流速1.0 mL·min-1，波长230 nm，流动相乙腈-0.05%磷酸水溶液，以芍药苷为参照峰，评价10批芍药汤HPLC指纹图谱。结果：确定了其中13个共有峰，并对其中的3个峰进行指认，分别为芍药苷、黄芩苷、小檗碱，建立了芍药汤水煎液指纹图谱；10批芍药汤HPLC指纹图谱相似度均>0.96。结论：该法灵敏度高、稳定性和准确性良好，体现了芍药汤组分的整体特征，为芍药汤制剂质量标准的建立提供了可靠的科学依据。

Abstract

Objective: To establish the chromatographic fingerprint for Shaoyao Tang by RP-HPLC. Method: The fingerprint was determined by Agilent ZORBAX SB-C18 column (4.6 mm×250 mm

5 μm)

with the column temperature 25℃

the flow rate 1.0 mL·min-1

the detective wavelength 230 nm and gradient elution with acetoniteile-0.05% phosphoric acid as mobile phase. Using paeoniflorin peak as reference

the HPLC fingerprints of 10 batches of Shaoyao Tang were analyzed. Result: Thirteen common peaks were marked and attributed to the herbs

and 3 common peaks were determined by the relative time of the standard substance

they are paeoniflorin

baicalin and berberine respectively. The HPLC fingerprint of Shaoyao Tang was established and the similarity of the 10 batches of samples was above 0.96. Conclusion: The established HPLC-DAD fingerprint is accurate

stable and reproducible

which could reflect the overall characteristics of Shaoyao Tang and improve the specificity for quality standard of the Chinese herbal formula.

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