Objective: To observe the neuroprotective effects of cerebral ischemic preconditioning (CIP) on cerebral ischemia reperfusion and the expressions of brain-derived neurotrophic factor(BDNF)

grial cell-lime derived neurotrophic factor(GDNF) and vascular endothelial growth factor(VEGF) in the cortex and hippocampus CA1 area of rats. Method: Wistar rats were randomly divided into three groups

Sham operation group

cerebral ischemia reperfusion (I/R) group

and cerebral ischemic tolerance (CIP+MCAO) group. The behavioral changes were detected by neurologic deficit scores (NDS)

the cerebral histopathology was detected by HE staining

and the level of NSE in serum were detected by ELISA. The expressions of BDNF

GDNF and VEGF in cortex

hippocampus CA1 area were measured by immunohistochemical staining. Result: Compared with Sham operation group

I/R group showed significant increases in neurologic deficit scores and the level of NSE in serum

and improved cerebral histopathology injury (P<0.01)

notable decreases in positive area and integral absorbance (IA) in cortex and hippocampus CA1 area at the damage side

but only statistical differences between brain cortex and Sham operation group; Compared with I/R group

CIP+MCAO decreased the neurologic deficit scores (P<0.05)

cerebral histopathology injury (P<0.05)

and NSE in serum (P<0.01). At the same time

the positive area and integral absorbance (IA) of BDNF

VEGF in cortex

and CA1 area at the damage side increased obviously (P<0.01

P<0.05). The positive expression of GDNF showed an increasing trend

but with no statistical difference. Conclusion: The neuroprotective effects of CIP were correlated with the up-regulation of the endogenous proteins BDNF and VEGF.