苷泰胶囊对四氯化碳诱导的大鼠肝纤维化的治疗作用

李光全; 刘慧敏; 崔鹤蓉; 张振芳; 桑秀秀; 王家葵; 柏兆方

doi:10.13422/j.cnki.syfjx.2016180127

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苷泰胶囊对四氯化碳诱导的大鼠肝纤维化的治疗作用

药理 | 更新时间：2024-01-04

- 苷泰胶囊对四氯化碳诱导的大鼠肝纤维化的治疗作用
- Therapeutic Effect of Gantai Capsules on CCl-induced Liver Fibrosis in Rats
- 中国实验方剂学杂志 2016年22卷第18期页码：127-131
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81330090）
- DOI：10.13422/j.cnki.syfjx.2016180127
  中图分类号： R259
- 纸质出版日期：2016
- 稿件说明：
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李光全, 刘慧敏, 崔鹤蓉, 等. 苷泰胶囊对四氯化碳诱导的大鼠肝纤维化的治疗作用[J]. 中国实验方剂学杂志, 2016,22(18):127-131.

LI Guang-quan, LIU Hui-min, CUI He-rong, et al. Therapeutic Effect of Gantai Capsules on CCl-induced Liver Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 127-131.
李光全, 刘慧敏, 崔鹤蓉, 等. 苷泰胶囊对四氯化碳诱导的大鼠肝纤维化的治疗作用[J]. 中国实验方剂学杂志, 2016,22(18):127-131. DOI： 10.13422/j.cnki.syfjx.2016180127.

LI Guang-quan, LIU Hui-min, CUI He-rong, et al. Therapeutic Effect of Gantai Capsules on CCl-induced Liver Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 127-131. DOI： 10.13422/j.cnki.syfjx.2016180127.

摘要

目的：研究苷泰胶囊对四氯化碳（CCl4）诱导的大鼠肝纤维化的治疗作用，并探讨其相应的作用机制。方法：SD大鼠将连续8周ip CCl4成功诱导的肝纤维化大鼠随机分为模型组，阳性药组秋水仙碱（2 mg·kg-1），苷泰胶囊低、高剂量组（230，460 mg·kg-1），每组12只，另设同批次正常大鼠12只为正常组。除正常组和模型组给予等体积生理盐水外，各组均按给定剂量ig给药，每日1次，连续4周。给药结束后处死大鼠，取血取肝。采用比色法测定血清中丙氨酸氨基转移酶（ALT），天门冬氨酸氨基转移酶（AST）的活性水平；采用酶联免疫吸附测定（ELISA）法测定透明质酸（HA），层粘蛋白（LN），Ⅳ型胶原（CⅣ）和Ⅲ型前胶原（procollagen Ⅲ，PCⅢ）的含量；采用碱水解法处理肝组织后测定其羟脯氨酸（HyP）的含量；同时采用苏木素-伊红（HE）和马松（Masson）染色法对肝组织染色，并于光学显微镜下观察比较肝脏病理学变化。结果：与正常组比较，模型组大鼠血清中ALT，AST，HA，LN，CⅣ和PCⅢ的水平明显升高，肝组织中HyP的含量明显升高（P<0.01），病理学结果显示模型组肝脏病理损伤程度较为明显，肝脏中胶原沉积较多；与CCl4模型组比较，苷泰胶囊能显著降低血清中ALT，AST，HA，LN，CⅣ和PCⅢ的水平（P<0.05，P<0.01），同时可使肝组织中HyP的含量明显下降（P<0.05，P<0.01）。病理学结果显示苷泰胶囊能明显改善肝脏病理损伤程度，减少肝脏中胶原沉积。结论：苷泰胶囊能够有效地减轻CCl4诱导的大鼠肝纤维化程度。

Abstract

Objective: To observe the therapeutic effects of Gantai capsules(GTC) on carbon tetrachloride(CCl4) induced liver fibrosis in rats

and investigate its action mechanism. Method: Liver fibrosis in rats was induced by intraperitoneal injection of CCl4 for 8 weeks. The SD rats were randomly divided to model group

the positive colchicine group(2 mg·kg-1)

and GTC low dose and high dose groups(230

460 mg·kg-1)

n=12 in each group. In addition

another 12 rats were selected from the same batch as normal group. The rats in normal and model groups were administrated with an equal volume of normal saline

while the rats in other groups were treated with corresponding substances at a given dose byig administration

1 time/day for 4 weeks. At the end of this experiment

all rats were sacrificed

and their blood and liver tissues were obtained for research. The activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum were detected by colorimetry; the levels of hyaluronic acid(HA)

laminin(LN)

procollagen Ⅲ(PC-Ⅲ) and collagen IV(CIV) in serum were detected by ELISA; the contents of hydroxyproline(HyP) in liver tissues were detected after alkali-hydrolysis; the liver tissues were stained by using HE and Masson staining; histopathological changes were observed under optical microscope. Result: As compared with the normal group

the levels of ALT

AST

CⅣ and PCⅢ in serum were significantly increased in model group; content of HyP in liver tissues was significantly increased(P<0.01); the liver pathological injury was obvious with more collagen deposition in the liver tissues. As compared with the model group

the levels of ALT

AST

CⅣ and PCⅢ in serum were significantly decreased in GTC groups(P<0.05

P<0.01); the contents of HyP in liver tissues was decreased(P<0.05

P<0.01). Pathological results showed that GTC could obviously improve the liver pathological injury and reduce collagen deposition in the liver tissues. Conclusion: GTC could effectively attenuate the CCl4-induced liver fibrosis in rats.

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