Objective: To investigate the clinical effect of Hehe Shuyang formula(HHSYF) in treatment of carbon tetrachloride (CCl4) induced hepatic injury and liver fibrosis in rats

and provide experimental basis for the clinical treatment of liver fibrosis. Method: By orthogonal test

40 SD rats were randomly divided into four groups: normal group

model group

positive drug group(bifendate

50 mg·kg-1·d-1)

and HHSYF group(83 g·kg-1·d-1). Chronic restraint stress+excessive exercise+improper diet were used in the normal group for modeling for 4 weeks

and then ig administration of distilled water to week 8. In the positive drug group

bifendate was given by ig administration till week 8; and ig administration was given in HHSYF group till week 8. Alanine aminotransferase(ALT)

aspartate aminotransferase(AST)

γ-glutamyltransferase(GGT) levels in serum and malondialdehyde(MDA) levels in liver tissues

as well as the level of hydroxyproline(HyP) were detected; histopathological observation was done by HE staining. Result: As compared with the normal group

the levels of ALT

AST and GGT in serum were significantly increased

and the levels of MDA and HyP in liver tissues were significantly increased in rats of model group(P<0.01); pathological results showed that the liver tissue injury was more obvious in model group. As compared with the model group

the levels of ALT

AST and GGT in serum were significantly decreased

and the levels of MDA and HyP in liver tissues were significantly decreased in rats of HHSYF group(P<0.01)

and the liver tissue injury was significantly improved. Conclusion: HHSYF has better effect on improving liver function and reversing hepatic fibrosis.