Objective: To obtain sustained-release preparations of ferulic acid liposomes by optimizing its formulation and technology. Method: The content of ferulic acid in liposomes was determined by HPLC.Ferulic acid was encapsulated into liposomes by calcium acetate gradient method

dialysis method was used to investigate release of ferulic acid.The factors such as phospholipids

the content of cholesterol

particle size and the addition of blood components were intensively investigated.Moreover

thermosensitive hydrogel technique was further applied to obtain the satisfactory sustained-release effect. Result: Hydrogenated soybean phosphatidylcholine (HSPC) was selected as the phospholipid composition. Optimum molar ratio between HSPC and cholesterol was determined at 4:1.Optimum average size of liposomes was found to be 397.7 nm.Blood components could accelerate the release of ferulic acid liposomes and there was little difference among different blood (human serum

fetal calf serum and rat plasma).Moreover

with the application of thermosensitive hydrogel technique

sustained-release effect was obtained in 48 h and the kinetics was fitted with Higuchi equation. Conclusion: Fetal calf serum and rat plasma can be used in drug release studies of liposomes to mimic human serum.Optimization of formulation and technique factors as well as the combined application of thermosensitive hydrogel technique can result in the satisfactory sustained-release properties of ferulic acid liposomes.