Objective: To compare specific HPLC fingerprints of Atractylodis Rhizoma before and after being processed with bran

and clear differences among characteristic peaks. Method: n-Hexane extracts of ten batches of raw products and the corresponding processed products with bran were analyzed by HPLC

mobile phase was acetonitrile-water for gradient elution

the detection wavelength was 254 nm.Taking atractylodin as the reference peak

common pattern of HPLC fingerprints about raw and processed products was constructed. Result: There were 26 common peaks in HPLC of raw and processed products of Atractylodis Rhizoma

the relative retention time of common peaks was almost the same with RSD <0.5%;but difference of their relative peak area was obvious.In HPLC fingerprints

the area ratio of chromatographic peaks at 47.36 min to 46.35 min was greater than 1 in raw products

but it was less than 1 in processed products;at the same time

the area ratio of peaks at 51.70 min to 49.25 min was less than 1 in raw products

but it was greater than 1 in processed products. Conclusion: Difference of chromatographic peaks in raw and processed products of Atractylodis Rhizoma is significant

and the results are stable with good specificity.It can be used as qualitative identification between raw and processed products of Atractylodis Rhizoma.