Objective: To evaluate the anti-platelet aggregation effect of horminone and 7-O-acetylhorminone in roots of Salvia deserta

and to establish a method for simultaneous determination of three effective constituents(horminone

7-O-acetylhorminone and 6

7-dehydroroyleanone) in S. deserta roots. Method: In vitro anti-platelet aggregation of horminone and 7-O-acetylhorminone was determined by turbidimetry. The contents of horminone

7-O-acetylhorminone and 6

7-dehydroroyleanone were simultaneously determined by HPLC. Result: As compared with the normal saline group

horminone and 7-O-acetylhorminone of the high and middle dose groups could effectively inhibit platelet aggregation induced by thrombin in a clear dose-dependent manner. The horminone

7-O-acetylhorminone and 6

7-dehydroroyleanone showed good linear relationship with the peak area within 0.31-4.56 μg(r=0.999 6)

0.01-0.24 μg(r=0.999 4) and 0.4-6.0 μg(r=0.999 5); their average recoveries were 100.3%(RSD 2.0%)

100.6%(RSD 3.1%)

and 102.5%(RSD 1.6%) respectively. Conclusion: Horminone and 7-O-acetylhorminone in roots of S. deserta showed better anti-platelet aggregation effect. The method of HPLC is simple

accurate and repeatable foe content determination

and can be used for quality control and evaluation of S. deserta.