Objective: To establish an HPLC method for simultaneous determination of 9 illegally added anti-diabetic chemical components in Chinese patent medicines. Method: The compounds were separated on C18 column

with methanol-0.1 mol·L-1 ammonium acetate solution as the mobile phase for gradient elution. The detection wavelength was set at 235 nm and the column temperature was 35℃

with the flow rate of 1.0 mol·L-1. Full wavelength scanning was from 190 nm to 400 nm. Suspicious ingredients were confirmed by mass spectrometry. Result: All 9 components had good linear relationship within their respective concentration range(r≥0.999 9)

 and their precision

stability

limit of detection

limit of quantitation and recovery rate met the standards. Illegally added glyburide was detected in Jiangtangning. Conclusion: The method is simple

accurate

and suitable for the determination of 9 illegally added anti-diabetic chemical components above.