Objective: To investigate effect of Glycyrrhizae Radix et Rhizoma on metabonomics of triptonide

find toxicity biomarkers and attenuation biomarkers induced by Glycyrrhizae Radix et Rhizoma and disclose toxicity and attenuation mechanism of Glycyrrhizae Radix et Rhizoma on triptonide. Method: SD rats were randomly divided into three groups of the control group

the triptonide group (dose of 0.7 mg·kg-1) and the triptonide (dose of 0.7 mg·kg-1)+Glycyrrhizae Radix et Rhizoma (dose of 3 mL·kg-1) group.All drug treatments were administered daily for consecutive 7 days.All urine samples were determined by UPLC-Q-TOF-MS.These obtained data were analyzed by principal component analysis (PCA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA). Result: Seven biomarkers related to toxicity of triptonide were found

four of them were identified as pantothenic acid

kynurenic acid

tryptophan and coproporphyrin

respectively.Four attenuation biomarkers were identified

one of them was tryptophan

but these remaining three biomarkers were unknown. Conclusion: These toxicity biomarkers and attenuation biomarkers are identified for the first time.It is indicated that toxicity of triptonide interfered with the energy

tryptophan and porphyrin metabolisms in body

recover of these metabolisms induced by Glycyrrhizae Radix et Rhizoma may be a physiological attenuation mechanism of Glycyrrhizae Radix et Rhizoma on triptonide.