Objective: To investigate the effect of Biejiajian Wan (BP) on angiogenesisand the structure of the new vessels in Matrigel implant of mice induced by basic fibroblast growth factor(b-FGF). Method: Totally 32 Balb/c mice were selected

and angiogenesis model was established by matrigel implant assay. Matrigel (500 μL) containing b-FGF and heparin was subcutaneously transplanted into the abdomens of mice

and then the mice were randomly divided into BP high dose and low dose groups (12

6 mg·kg-1)

normal group

endostatin group (5 mg·kg-1)

n=8 in each group. The corresponding drugs were given 3 days before Matrigel implant for 8 days. Matrigel was taken out at the eighth day

and hemoglobin content in Matrigel implant was detected; HE staining and immunohistochemical staining were used to detect microvessel density count (MDC); meanwhile

the structure characteristics and development stage of the new vessels in matrigel implant were observed and compared. Result: Hemoglobin content and MDC in high and low dose BP groups were lower than those in normal group; in addition

the hemoglobin content and MDC in low dose BP group were lower than those in high dose group. HE staining results showed some scattered new vessels in low dose and high dose BP groups

and the number of new vessels was lower than that in normal group. Furthermore

CD31 immunohistochemical staining results showed that the number of endothelial cells in BP groups was lower than that in normal group; the structure of the new vessels became fuzzy and the endothelial cells became lighter in color as compared with the control group. Conclusion: BP have inhibitory effect on angiogenesis in a concentration-dependent manner; low dose BP has a stronger inhibitory effect than high dose BP on angiogenesis.