QU Yong-sheng, XIE Ying-ying, GUO Wei, et al. Optimization of Extraction Process of Prunellae Spica by Box-Behnken Response Surface Methodology and Micro-genetic Algorithm[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(20): 14-17.
QU Yong-sheng, XIE Ying-ying, GUO Wei, et al. Optimization of Extraction Process of Prunellae Spica by Box-Behnken Response Surface Methodology and Micro-genetic Algorithm[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(20): 14-17. DOI: 10.13422/j.cnki.syfjx.2016200014.
Objective: To optimize ultrasonic extraction process ranges of rosmarinic acid from Prunellae Spica. Method: Taking ethanol concentration
liquid-solid ratio and extraction time as factors
yield of rosmarinic acid as index
mathematical model was established by Box-Behnken response surface methodology.The model was optimized by micro-genetic algorithm in order to obtain the optimum ranges of extraction process parameters. Result: Optimal conditions of extraction process were as following:ultrasonic power of 200 W
ultrasonic frequency of 53 kHz
ethanol concentration of (50.03±1.21)%
liquid-solid ratio of (48.28±2.27) mL·g-1
extraction time of (47.34±1.43) min.Yield of rosmarinic acid was 0.301%. Conclusion: This optimized process has a high yield of rosmarinic acid
it can obtain a floated wide of process parameters.