Objective: To investigate the effects of Fufang Lujiao Rutongning capsule in relieving hyperplasia of mammary gland and pain in rats with mammary gland hyperplasia

and discuss its relevant action mechanisms. Method: In the experiment of anti-hyperplasia of mammary glands

the model of mammary gland hyperplasia was induced by estradiol(E2) and progesterone

in order to evaluate the protein expression of sex hormone receptors in breast issues and the serum levels of sex hormone. In the analgesic experiment

glacial acetic acid was used to induce writhing response

and photothermal radiometry was adopted to cause tail flick

in order to evaluate the analgesic effect of Fufang Lujiao Rutongning capsule. Result: Compared with the model group

Fufang Lujiao Rutongning capsule could markedly reduce the protein expressions of estrogen receptor and progesterone receptor in mammary tissues of rats with mammary gland hyperplasia

and the percentage of positive expression cells in similar cells (P<0.01); high and middle-dose group markedly decrease E2 and increased corporin in rat serum (P<0.05

P<0.01). In addition

Fufang Lujiao Rutongning capsule high and middle-dose groups could significantly prolong the incubation period of body torsion reaction (P<0.01)

reduce the body torsion reaction times (P<0.01)

and significantly shorten the pain threshold in rats induced by photothermal radiometry at 60

120

180

240 min after the treatment (P<0.01). Conclusion: Fufang Lujiao Rutongning capsule has the anti-mammary gland effect by regulating sex hormone levels

with a remarkable analgesic effect.