Objective: To study of the effect and mechanism of Erdong Gao on gastric cancer rats through the analysis on cell apoptosis and bio-marker. Method: The rats was divided into normal group

model group

retinoic acid (12×10-3g·kg-1) group

and low-dose and high-dose Erdong Gao (6

12 g·kg-1) groups. Except for normal group

the model was made through gavage with N-methy-N'-nitro-N-nitrosoguanidine (MNNG) 0.2 g·kg-1

once every 10 days

for a total of 9 times. Each group was given corresponding drugs through gavage

and the normal group was given isopyknic NS. After 3 months

rats ware sacrificed

the pathological changes were observed by hematoxylin-eosin(HE) staining

the mRNA expression of Bcl-2 was detected by Real-time PCR

the cell apoptosis was detected by TdT-mediated dUTP nick end labeling (TUNEL)

and the plasma metabonomics was performed to find differentiated metabolites. Result: Different does of Erdong Gao can improve the pathological changes of gastric cancer

mainly by targeting at the benign papillary hyperplasia and papilloma; low-dose Erdong Gao can significantly suppress the mRNA expression of Bcl-2 (P<0.01). According to TUNEL

high and low-dose Erdong Gao groups showed a higher rate of cell apoptosis than the model group. Plasma metabolites of low-dose Erdong Gao showed Sphingosine-1-phosphate

20-hydroxyeicosatetraenoic acid (20-HETE)

and glycocholic acid

which were correlated with cell apoptosis. Conclusion: Erdong Gao could promote cell apoptosis in gastric cancer rats.