贞芪扶正胶囊对再生障碍性贫血大鼠EPO,IL-2,IL-11及CD34细胞的影响

刘现辉; 郭晓娜

doi:10.13422/j.cnki.syfjx.2016200143

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贞芪扶正胶囊对再生障碍性贫血大鼠EPO,IL-2,IL-11及CD34细胞的影响

药理 | 更新时间：2024-01-04

- 贞芪扶正胶囊对再生障碍性贫血大鼠EPO,IL-2,IL-11及CD34细胞的影响
- Effect of Zhenqi Fuzheng Capsule on EPO, IL-2, IL-11,CD34 Cells in Aplastic Anemia Rats
- 中国实验方剂学杂志 2016年22卷第20期页码：143-147
- 作者机构：
- 作者简介：
- 基金信息：
  
  河南省中管局重点专科（学科）学术带头人培养项目（121PCXTD510）
- DOI：10.13422/j.cnki.syfjx.2016200143
  中图分类号： R285.5
- 纸质出版日期：2016
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刘现辉, 郭晓娜. 贞芪扶正胶囊对再生障碍性贫血大鼠EPO,IL-2,IL-11及CD34细胞的影响[J]. 中国实验方剂学杂志, 2016,22(20):143-147.

LIU Xian-hui, GUO Xiao-na. Effect of Zhenqi Fuzheng Capsule on EPO, IL-2, IL-11,CD34 Cells in Aplastic Anemia Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(20): 143-147.
刘现辉, 郭晓娜. 贞芪扶正胶囊对再生障碍性贫血大鼠EPO,IL-2,IL-11及CD34细胞的影响[J]. 中国实验方剂学杂志, 2016,22(20):143-147. DOI： 10.13422/j.cnki.syfjx.2016200143.

LIU Xian-hui, GUO Xiao-na. Effect of Zhenqi Fuzheng Capsule on EPO, IL-2, IL-11,CD34 Cells in Aplastic Anemia Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(20): 143-147. DOI： 10.13422/j.cnki.syfjx.2016200143.

摘要

目的：观察贞芪扶正胶囊对再生障碍性贫血（AA）模型大鼠重组人红细胞生成素（EPO），CD34+细胞，白细胞介素-2（IL-2），白细胞介素-11（IL-11）的影响，探讨其相关的作用机制。方法：按随机数字将60只清洁级Wistar大鼠分为6组，分别为正常组、模型组、贞芪扶正胶囊低、中、高剂量组（5，10，20 g·kg-1）及阳性药组（司坦唑醇混悬液，0.004 g·kg-1），除正常组外，其余均釆用5-氟尿嘧啶（5-FU）与马利兰联合建立大鼠AA模型。模型成功后，给药组给予相应药物给药，阳性药组给予司坦唑醇混悬液，正常组与模型组给予相同体积的生理盐水，连续ig 30 d，以外周血细胞计数，骨髓单个核细胞（BMNC）计数，酶联免疫吸附测定（ELISA）法检测IL-2，IL-11，EPO，肿瘤坏死因子-α（TNF-α）水平，CD34+抗原和Fas抗原检测为主要观察指标综合评价贞芪扶正胶囊的干预效果。结果：与正常组比较，模型组的外周血白细胞（WBC），血小板（PLT），红细胞（RBC）及血红蛋白（Hb），BMNC，IL-11，CD3+T细胞比例，CD3+CD4+T细胞比例，CD34+抗原荧光量均明显降低，IL-2，EPO，TNF-α，Fas抗原荧光量明显升高（P<0.01）；与模型组比较，司坦唑醇组、贞芪扶正胶囊高剂量组的WBC，RBC，PLT，Hb，BMNC均有所升高（P<0.05，P<0.01），贞芪扶正胶囊中、高剂量组的IL-11，CD3+T细胞，CD3+CD4+T细胞比例，CD34+抗原荧光量升高，IL-2，EPO，TNF-α，Fas抗原荧光量降低降低（P<0.05，P<0.01）；与司坦唑醇组比较，贞芪扶正胶囊中、高剂量组的WBC，CD3+T细胞比例，CD3+CD4+T细胞比例，IL-11，CD34+抗原荧光量较高（P<0.05），IL-2，TNF-α，Fas抗原荧光量较低（P<0.05）。结论：贞芪扶正胶囊能够改善AA大鼠外周血细胞状况、骨髓造血组织功能的恢复，增强免疫功能，可能与调控因子EPO，IL-2，IL-11水平和CD34+细胞密切相关。

Abstract

Objective: To observe the effect of Zhenqi Fuzheng capsule on erythrogenin(EPO)

CD34+ cells

interleukin-2(IL-2)

IL-11 in aplastic anemia rats. Method: Totally 60 clean-grade Wistar rats were randomly divided into 6 groups:normal group

model group

Zhenqi Fuzheng capsule low

middle and high-dose groups (5

20 g·kg-1) and positive drug group (stanozolol suspension

0.004 g·kg-1). Except for the normal group

the other groups were given 5-fluorouracil (5-FU) combined with Maryland to establish aplastic anemia (AA) rats model. After successful modeling

Zhenqi Fuzheng capsule groups were given Zhenqi Fuzheng capsule

the positive drug group was given Stanozolol suspension

and the normal group and the model group were given the same volume of normal saline

for 30 days in a row. Peripheral blood cells and bone marrow monouclear cells(BMNCs) were counted. EPO

IL-2

IL-11

and tumor necrosis factor-α(TNF-α) were detected by ELISA

with CD34+ antigens and Fas antigens as the main index for the comprehensive evaluation on the intervention effect of Zhenqi Fuzheng capsule. Result: Compared with the normal group

WBC

RBC

PLT

BMNC

IL-11

proportion of CD3+T cells

proportion of CD3+ CD4+T cells

CD34+ antigen fluorescent volume of the model group were significantly lower

IL-2

EPO

TNF-α

Fas antigen fluorescent quantity increased significantly (P<0.01). Compared with the model group

WBC

RBC

PLT

Hb and BMNC cell count in the Stanozolol group

and Zhenqi Fuzheng capsule middle-dose and high-dose groups were increased (P<0.05

P<0.01)

IL-11

CD3+T cells

proportion of CD3+ CD4+T cells

CD34+ antigen fluorescent quantity in Zhenqi Fuzheng capsule middle-dose and high-dose groups increased

IL-2

EPO

TNF-α

Fas antigen fluorescence decreased (P<0.05

P<0.01). Compared with the Stanozolol group

WBC

proportion of CD3+T cells

CD3+

CD4+T cell percentage

IL-11

CD34+ antigen fluorescence quantity in Zhenqi Fuzheng capsule middle-dose and high-dose groups were higher (P<0.05)

IL-2 and TNF-α

Fas antigen fluorescent quantity were lower (P<0.05). Conclusion: Zhenqi Fuzheng capsule can improve peripheral blood cells

hematopoietic function and immune function of AA rats. It may be closely related with regulatory factors EPO

IL-2

IL-11 levels and CD34+ cells.

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