基于主成分分析、聚类分析和典型相关分析的漏芦抗胃癌谱效关系探索

于海帅

doi:10.13422/j.cnki.syfjx.2016210027

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基于主成分分析、聚类分析和典型相关分析的漏芦抗胃癌谱效关系探索

药剂与炮制 | 更新时间：2024-01-04

- 基于主成分分析、聚类分析和典型相关分析的漏芦抗胃癌谱效关系探索
- Research on Spectrum-activity Relationship of Echinopsis Radix for Anti-gastric Cancer Based on Principal Component Analysis, Cluster Analysis and Canonical Correlation Analysis
- 中国实验方剂学杂志 2016年22卷第21期页码：27-31
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- DOI：10.13422/j.cnki.syfjx.2016210027
  中图分类号： O657.72;R284
- 纸质出版日期：2016
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于海帅. 基于主成分分析、聚类分析和典型相关分析的漏芦抗胃癌谱效关系探索[J]. 中国实验方剂学杂志, 2016,22(21):27-31.

YU Hai-shuai. Research on Spectrum-activity Relationship of Echinopsis Radix for Anti-gastric Cancer Based on Principal Component Analysis, Cluster Analysis and Canonical Correlation Analysis[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 27-31.
于海帅. 基于主成分分析、聚类分析和典型相关分析的漏芦抗胃癌谱效关系探索[J]. 中国实验方剂学杂志, 2016,22(21):27-31. DOI： 10.13422/j.cnki.syfjx.2016210027.

YU Hai-shuai. Research on Spectrum-activity Relationship of Echinopsis Radix for Anti-gastric Cancer Based on Principal Component Analysis, Cluster Analysis and Canonical Correlation Analysis[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 27-31. DOI： 10.13422/j.cnki.syfjx.2016210027.

摘要

目的：研究漏芦化学成分和抗胃癌药效之间的谱效关系。方法：采用HPLC分析7种产地漏芦的化学成分，对共有峰进行主成分分析，根据相关系数矩阵、特征值和累计方差贡献率，初步推断漏芦的主成分；采用噻唑蓝法检测7种产地漏芦抑制胃癌细胞SGC-7901和AGS增殖的作用，对7批漏芦进行聚类分析；利用典型相关分析对漏芦成分、药效数据进行相关分析。结果：在漏芦17个共有峰中，主成分为迷迭香酸、齐墩果酸和豆甾醇。7个产地漏芦抑制SGC-7901细胞和AGS细胞增殖的作用顺序均为河南 >山东 >辽宁 >山西 >安徽 >江苏 >甘肃。聚类分析显示河南和山东产地为1类，辽宁和山西产地为1类，安徽、江苏和甘肃产地为1类。结论：河南产地的漏芦抑制胃癌细胞SGC-7901和AGS增殖的作用最强，漏芦抗胃癌的主成分可能为迷迭香酸、齐墩果酸、豆甾醇等。

Abstract

Objective: To study on spectrum-activity relationship between chemical composition and anti-gastric cancer efficacy of Echinopsis Radix in Yuzhou. Method: Chemical composition in Echinopsis Radix from seven regions was analyzed by HPLC

and principal component analysis was carried out for its comom peak

according to correlation coefficient matrix

characteristic value and accumulated variance contribution rate

principal components of Echinopsis Radix were inferred.MTT was used to assay proliferation inhibition rate of Echinopsis Radix from seven regions for gastric cancer cell line SGC7901 and AGS

then for cluster analysis.Canonical correlation analysis was used to analyze relationship between chemical composition and anti-gastric cancer efficacy of Echinopsis Radix. Result: Principal components of Echinopsis Radix were rosmarinic acid

oleanolic acid and stigmasterol in seventeen common peaks.Proliferation inhibition rates of Echinopsis Radix from different region on SGC-7901 and AGS cell were in the order of Henan >Shandong >Liaoning >Shanxi >Anhui >Jiangsu >Gansu.Cluster analysis showed that Henan and Shandong classified the same species

Liaoning and Shanxi classified the same species

Anhui

Jiangsu and Gansu classified the same species. Conclusion: Echinopsis Radix from Henan has the strongest proliferation inhibition effect on gastric cancer cell line SGC7901 and AGS.Rosmarinic acid

oleanolic acid and stigmasterol in Echinopsis Radix may be these principal components for anti-gastric cancer.

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