Objective: To study the chemical constituents from rhizome of Athyrium multidentatum and their reducing capacity. Method: The chemical constituents were separated and purified by silica-gel column chromatography

and identified according to physicochemical properties and spectral data. The reducing capacity was detected by ultraviolet-visible spectrophotometry. Result: Eleven compounds were obtained from A. multidentatum rhizome and identified as methyl linoleate (1)

physcion (2)

tetracosanoic acid (3)

1-hexacosanol (4)

linoleic acid (5)

β-sitosterol (6)

daucosterol (7)

striatisporolide A (8)

protocatechuic acid methyl ester (9)

protocatechuic acid (10) and D-glucose (11). Conclusion: Except for compounds 3 and 6

all of the remaining compounds were prepared from A. multidentatum for the first time

and compounds 9 and 10 showed a notable reducing capacity.