Objective: Studies on the chemical constituents of Guangxi characteristic medicine Murdannia nudiflora. Method: The slica column chromatography and recrystallization were used to isolate and purify the chemical constituents of M. nudiflora. Result: Fifteen compounds have been isolated and identified as:n-nonatriacontane(1)

n-octyl eicosenoate (2)

cholest-7-en-3-one (3)

22

23-dihydrospinasterone (4)

n-tetracosanoic acid (5)

n-triaconatanoic acid (6)

β-sitosterol (7)

campesterol (8)

stigmasterol (9)

ergostenol (10)

stigmast-7-en-3-ol (11)

stigmasterol-3-O-glucopyranoside (12)

daucosterol (13)

(2S)-1-O-heptatriacontanoyl glycerol (14)

bracteanolide A (15). Conclusion: Compounds 1-15 were isolated from this plant for the first time.