Objective: To research the anti-hepatic fibrosis effect of ammonium glycyrrhetate combined oxymatrine in hepatic fibrosis rat induced by CCl4 and its mechanism. Method: SD rats were randomly divided into six groups

namely normal group

model group

colchicine positive group (2 mg·kg-1)

ammonium glycyrrhetate group (15 mg·kg-1)

oxymatrine group (30 mg·kg-1)

ammonium glycyrrhetate combined oxymatrine group (15 mg·kg-1+30 mg·kg-1)

with 10 in each group. Except for normal group

in the remaining groups

the rat liver fibrosis model were induced by CCl4.Normal group and model group were given the saline

treatment groups were administered with the corresponding drugs of equal clinical doses. Their liver fibrosis were assessed by detecting serum alanine transaminase (ALT)

aspertate aminotransferase (AST) and liver hydroxyproline (Hyp) and observing liver tissue pathology biopsy. Besides

serum interleukin-6(IL-6)

acetylcholin esterase (AchE)

high mobility group protein 1 (HMGB1)

and lipopolysaccharide (LPS) were also detected. Result: Compared with model group

model group showed remarkable increases in ALT

AST

IL-6

AchE

LPS

HMGB1 and liver Hyp (P<0.01)

significant liver tissue pathological changes

and notable expansion in collagen area. Compared with model group

ALT

AST and IL-6

AchE

LPS

HMGB1 levels and liver Hyp significantly decreased in treatment group (P<0.05)

At the same time

compared with model group

oxymatrine group showed obvious decrease in the expression of AchE (P<0.05)

and combination group showed a higher AchE expressions than oxymatrine group (P<0.05)

and significant alleviation in liver tissue pathological changes and collagen area. Conclusion: Ammonium glycyrrhetate combined oxymatrine can inhibit CCl4-induced liver fibrosis in rats by reducing the levels of LPS and the expression of IL-6

HMGB1.The combined administration can improve the clinical effect in declining acetyl cholinesterase by using oxymatrine alone.