黄芩素对绒毛滋养细胞侵袭的影响

吕珊珊; 候莉莉

doi:10.13422/j.cnki.syfjx.2016210127

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黄芩素对绒毛滋养细胞侵袭的影响

药理 | 更新时间：2024-01-04

- 黄芩素对绒毛滋养细胞侵袭的影响
- Effect of Baicalein on Cell Invasion in Human Trophoblast Derived HTR8/SVneo Cells
- 中国实验方剂学杂志 2016年22卷第21期页码：127-131
- 作者机构：
- 作者简介：
- 基金信息：
  
  南京市医学科技发展计划项目（ZKX15047）
- DOI：10.13422/j.cnki.syfjx.2016210127
  中图分类号： R285
- 纸质出版日期：2016
- 稿件说明：
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吕珊珊, 候莉莉. 黄芩素对绒毛滋养细胞侵袭的影响[J]. 中国实验方剂学杂志, 2016,22(21):127-131.

LYU Shan-shan, HOU Li-li. Effect of Baicalein on Cell Invasion in Human Trophoblast Derived HTR8/SVneo Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 127-131.
吕珊珊, 候莉莉. 黄芩素对绒毛滋养细胞侵袭的影响[J]. 中国实验方剂学杂志, 2016,22(21):127-131. DOI： 10.13422/j.cnki.syfjx.2016210127.

LYU Shan-shan, HOU Li-li. Effect of Baicalein on Cell Invasion in Human Trophoblast Derived HTR8/SVneo Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 127-131. DOI： 10.13422/j.cnki.syfjx.2016210127.

摘要

目的：通过研究黄芩素对人类早孕期绒毛滋养细胞株HTR-8/SVneo的增殖活力、侵袭能力的影响，探讨中药黄芩作用于滋养细胞侵袭相关疾病的可能作用机制。方法：体外培养早孕期绒毛滋养细胞株HTR-8/SVneo细胞，以不同浓度（0.01，0.05，0.1，0.5，1 μmol·L-1）黄芩素作用于细胞，设黄芩素0 μmol·L-1为空白组，采用细胞增殖、毒性活力实验法（cell counting kit-8，CCK-8）检测黄芩素对HTR-8/SVneo细胞增殖活力的影响，细胞侵袭实验（Transwell）检测黄芩素对HTR-8/SVneo细胞侵袭作用的影响。提取黄芩素处理后的细胞总RNA，蛋白，分别运用实时荧光定量PCR（Real-time PCR），蛋白免疫印迹法（Western blot）检测滋养细胞侵袭相关的重要因素-基质金属蛋白酶（MMP）-2，MMP-9的mRNA，蛋白表达水平；收集细胞上清，培养基采用明胶酶谱法检测黄芩素对MMP-2，MMP-9的分泌及酶活性的影响。结果：与空白组比较，0.01，0.05，0.1，0.5 μmol·L-1黄芩素能明显诱导增强滋养细胞HTR-8/SVneo的侵袭能力（P<0.05），且侵袭率对剂量呈依耐性增长。0.01，0.05，0.1，0.5 μmol·L-1黄芩素可明显上调MMP-9蛋白和mRNA表达水平，增强其酶活性（P<0.05）。结论：黄芩素能诱导增强滋养细胞HTR-8/SVneo的侵袭能力，可能是通过增强MMP-9 mRNA和蛋白表达及酶活性来发挥作用。

Abstract

Objective: To investigated the effects of baicalein on proliferation activity and invasive ability of extravillous trophoblast derived HTR-8/SVneo cells

and explore the potential mechanism of Chinese medicine baicalein in treating trophoblast diseases such as recurrent spontaneous abortion (RSA). Method: HTR-8/SVneo cells were cultured in vitro

and with baicalein 0 μmol·L-1 as the blank group

the effect of different concentrations of baicalein (0.01

0.05

0.1

0.5

1 μmol·L-1) on cell proliferation activity was evaluated by cell counting kit-8 (CCK-8) assays. Transwell assay was proformed to evaluate the effect of baicalein on cell invasive ability. Total RNA and protein of HTR-8/SVneo cells exposured with baicalein (0.01

0.05

0.1

0.5

1 μmol·L-1) were isolated to assess the mRNA and protein levels of matrix metalloproteinase (MMP)-2

MMP-9 by Real-time PCR and Western blot respectively. At the same time

cell supernatant was collected to observe the effect of baicalein on secretion and enzyme activities of MMP-9

MMP-2 by gelatin zymography. Result: As compared with balnk group

0.01

0.05

0.1

0.5 μmol·L-1 baicalein could significantly increase the invasive ability of HTR-8/SVneo cells in a concentration-dependent manner (P<0.05).The 0.01

0.05

0.1

0.5 μmol·L-1 baicalein could significantly up-regulate the protein and mRNA expressions of MMP-9 and enhance its enzyme activities (P<0.05). Conclusion: Baicalein could induce to increase the invasive ability of trophoblast HTR-8/SVneo cells

probably by increasing the protein and mRNA expressions as well as enzyme activities of MMP-9.

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