Objective: To compare four different extraction methods for recycling wastes from traditional Chinese medicine production

in order to select the optimal method to extract polysaccharide from Mailuoning injection production waste Dendrobii Mobile dregs. Method: The four methods

namely wet ultrafine grinding method

enzyme method

ultrasonic method

heating reflux method

were adopted. The polysaccharide extraction rate was detected by phenol sulfuric acid colorimetry; the process with the highest extraction rate was determined by single factor and orthogonal test; the effect of Dendrobii Mobile dregs polysaccharide(DDP) on the proliferation and transformation of lymphocytes in mouse and the NO release of macrophage RAW264.7 were investigated. Result: The polysaccharide extraction rates of Dendrobii Mobile dregs by the above four different methods were 0.51%

8.79%

5.34%

3.44%

respectively. The optimal extraction method was determined to be the enzymolysis; its optimal enzymolysis conditions were pH at 5.3

solid-liquid ratio at 55

temperature at 50℃

enzyme dosage of 4 000 U·g-1

extraction time of 300 minutes. Compared with control group

DDP significantly enhanced the proliferation of splenic lymphocytes at concentrations between 62.5-125 mg·L-1

with an obvious synergistic effect between DDP and lipopolysaccharide(LPS) (P<0.05

P<0.01); at the concentration of 62.5 mg·L-1

DDP showed a significant synergistic effect with ConA (P<0.05); at concentrations between 62.5-500 mg·L-1

DDP played a notable role in promoting NO release of mouse macrophage RAW264.7 (P<0.01). Conclusion: Enzyme extraction process of polysaccharide from Dendrobii Mobile dregs was simple and stable

with the effect in immune enhancement

and worth clinical promotion.