Objective: To compare the contents of the active compositions from Zhizi Bopi Tang extracted by three methods of semi-bionic extraction method (SBE method)

alcohol extraction method (AE method)

water extraction method (WE method) and provide experimental evidence for extracting Zhizi Bopi Tang. Method: Zhizi Bopi Tang was extracted by three methods of SBE method

AE method and WE method under the same conditions in the size of decoction pieces

amount of solvent

and the same extraction

filtration and concentration equipment. The contents of gardenoside

berberine and glycyrrhetinic acid in the extracts under the above 3 methods were determined by HPLC method and the content of total alkaloids was determined by ultraviolet spectropho-tometric method. The comprehensive evaluation value Y was based on the weighted sum of processed data of gardenoside content

berberine content

glycyrrhetinic acid content

total alkaloids content and the extracts with molecular weight ≤1 000 Da

the samples with larger Y value were better. Result: The order of the comprehensive evaluation Y of the three methods was as follows:SBE extracting solution > AE extracting solution > WE extracting solution. Conclusion: It can be concluded that SBE method was the best one among the three methods for Zhizi Bopi Tang.