Objective: To compare pharmacokinetics of active constituents from Zhizi Dahuang Tang in normal and alcoholic liver injury (ALI) rats. Method: Rats were randomly divided into the normal group and the ALI group with 6 rats in each group.Rats were orally given Zhizi Dahuang Tang at a dose of 8 g·kg-1 (crude drug mass to body weight)

plasma samples were collected from the fosse orbital at different time point

UHPLC-MS/MS was adopted for the simultaneous determination of two iridoid glycosides (geniposide and genipin gentiobioside)

two anthraquinones (rhein and emodin) and four flavonoid glycosides (naringin

isonaringin

hesperidin and neohesperidin) in rat plasma with paeoniflorin as internal standard.After liquid-liquid extraction with ethyl acetate-isopropanol (1:1)

separation was achieved on a Shim-pack XR-ODS C18 column with a mobile phase consisting of water (containing 0.1% formic acid) and acetonitrile for gradient elution at a flow rate of 0.4 mL·min-1.Mass spectrometric detection was performed on 4000 QTRAP mass spectrometry equipped with turbo ion spray source in the negative ionization and multiple reaction monitoring (MRM) mode.The method was applied to compare the pharmacokinetic profiles of the analytes in normal and ALI rats after oral administration of Zhizi Dahuang Tang. Result: Compared with the normal group

a longer Tmax was observed for the eight analytes in the ALI group. In addition to rhein

t1/2 and MRT0-t extended in the ALI group; it was also found that Cmax

AUC0-t and AUC0-∞ of all analytes significantly increased

but clearance(CL) obviously decreased; however

rhein exhibited opposite results to the other seven analytes in all parameters excepted Tmax. Conclusion: There are remarkable differences in pharmacokinetic properties of the analytes from Zhizi Dahuang Tang between the normal and ALI group.