Objective: To explore pharmacokinetic characteristics of total alkaloids from Aconiti Lateralis Radix Praeparata with different dosage in rats

and discuss the relationship between the pharmacokinetics and the dosage. Method: Rats were randomly divided into the low

medium and high dosage groups by oral administration of total alkaloids from Aconiti Lateralis Radix Praeparata with the dosage of 9.6

19.2

38.4 mg·kg-1

respectively.A series of blood samples were collected through orbital venae angularis at time points of 0

5

10

20

30

40

60

120

180

300

420

540

720

1 440 min into heparinized tubes.The plasma samples were centrifuged and separated.UHPLC-Q-TOF-MS was established to detect blood drug concentrations of aconitine

mesaconitine

hypaconitine

benzoylaconitine

benzoylmesaconine

benzoylhypacoitine and aconine at different time points.Several concentration-time curves were drawn and pharmacokinetic parameters were calculated. Result: Compared with the low dosage group

Cmax and AUC0-∞ of seven alkaloids in the middle and high dosage groups increased

most of them had significant difference;the majority of Vz/F

CL/F

t1/2 and Tmax did not have significant difference. Conclusion: The dosage has a significant impact on pharmacokinetic characteristics of total alkaloids from Aconiti Lateralis Radix Praeparata in rats.In the range of low dosage to high dosage

the process in vivo of total alkaloids is basically in line with the linear dynamic characteristics.